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KMID : 0903619960370020317
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Journal of the Korean Society for Horticultural Science
1996 Volume.37 No. 2 p.317 ~ p.323
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Effects of Growth Regulators , Sucrose , Light , and Medium on callus and Shoot Formation in Shoot - tip Culture of Hibiscus syriacus L . ´ Honghwarang ´
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김기선/Kim, Ki Sun
유은하/유용권/Yoo, Eun Ha/Yoo, Yong Kweon
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Abstract
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This study was carried out to determine the optimum medium strength and cultural condition for callus formation and plant regeneration in shoot-tip culture of Hibiscus syriacus L. ´Honghwarang´. Shoot apex tissue was cultured to compare the effects of auxins, cytokinins, sucrose, light, and medium for callus induction. The induced calli were transferred to regeneration media containing kinetin, BAP, and TDZ, and then shoot regeneration was evaluated after 8 weeks. 2,4-D was better than NAA in callus formation and growth, with 2 ㎎·L^(-1) 2,4-D being the most effective. Cytokinins inhibited callus formation. One ㎎·L^(-1) kinetin or 0.5 ㎎·L^(-1) BAP were promotive for direct shooting from explant. Two percent sucrose was the most effective on callus formation, while higher concentration inhibited callus formation and growth. Light condition of 16/8 hr (L/D) was effective on callus formation and growth. The optimum medium for callus growth was 1/2MS. Subsequently, the best growth of callus, adventitious buds, and multiple shoots were obtained from MS medium containing 0.5 ㎎·L^(-1) TDZ, but the induced shoot did not elongate further. Development of induced shoots was better in 1 ㎎·L^(-1) BAP than TDZ. Consequently, in vitro mass production of Hibiscus syriacus L. can be obtained via indirect organogenesis through callus culure, which was derived from shoot tips at 2,4-D 2 ㎎·L^(-1), in the medium containing TDZ 0.5 ㎎·L^(-1).
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